MSH2 Knockout cell line (Hep G2)

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MSH2 Knockout cell line (Hep G2) Order

Catalog#

YKO-HT5101

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

MSH2 Knockout cell line (Hep G2)

Species

Gene Name

MSH2

Gene ID

4436

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the MSH2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, MSH2 gene knockout MSH2 Knockout cell line (Hep G2) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

MSH2 Knockout cell line (Hep G2)

Catalog#

YKO-HT5101

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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MSH2 Knockout cell line (Hep G2)

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